About Me
Hello! I am a versatile professional with skills in multiple domains. I enjoy learning and applying my knowledge to solve real-world problems. Click on a profession to see relevant experience!
Bachelors:
Biology
Biology
Dec 2018
University of New Haven
Masters: Cell and Molec. Biology
Jan 2020
University of New Haven
RA III
RA II
2020
Harvard University
Associate Scientist
Senior RA
2023
Intellia Therapeutics
Assay Development (NGS)
Assay Development (Cellular)
Immunologist
Data Scientist
CRISPR
Next-Generation Sequencing (NGS)
DNA Library Preparation
Illumina
High-Throughput Screening
Automation Hamilton
Automation Prime HighRes
PCR
Nucleofection / Electroporation
CRISPR
Pooled CRISPR Screens
Flow Cytometry (8 panel)
Cell Sorting, FACs (HSCs lineage-ckit+sca1+)
CD8 T cell
Cell Line Characterization
Murine Hematopoietic Stem Cells (HSCs LSKs)
Nucleofection / Electroporation
Murine Models
Cell Line Development (OVA expressing)
cytokine profiling
immune phenotyping
Cesium irradiation, husbandry, handle, care, and euthanasia
Lateral tail vein injections
Intra-peritoneal injections
Oral gavage
Subcutaneous tumor injections
Retro-orbital bleeding
Murine Spleen and Lymph Node Isolation (inguinal, brachial, axillary, cervical, mesenteric, and lumbar)
CRISPR
Functional Genomics
Nucleofection / Electroporation
Transfection
Transduction
Primary Human Hepatocytes (PHH)
Primary Human Hematopoietic Stem Cells (CD34+ HSCs)
HUDEP-2
Murine T Cells
Human PBMC processing
Various Cancer Cell Lines
Genome In a Bottle (LCLs from Coriell)
Western Blot
Machine Learning (ML)
Large Language Models (LLMs)
Data Modeling
Data Mining
Bulk RNA-Seq
Single Cell RNA-Seq
Python, ggplot2, Pandas, Matplotlib, Seaborn, Jupyter Notebook
R, DESeq2, CellRanger
Data Analysis
Visualization
VS Code
High Performance Computing with SLURM Scheduler
SQL
C
C++
Java
Git
Relevant Experience
Hover over a profession to see relevant experience here.
Projects
- TBD: Please wait, coming soon.
Publications and Patents
- LaFleur M.W., Lemmen A.M., Streeter I.S.L. et al. X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes in the immune system. Nat Immunol 25, 178–188 (2024). https://doi.org/10.1038/s41590-023-01689-6
- Milling L.E., Markson S.C., Tjokrosurjo Q., Derosia N.M., Streeter I.S.L. et al. Framework for in vivo T cell screens. J Exp Med 1 April 2024; 221 (4): e20230699. doi: https://doi.org/10.1084/jem.20230699
- LaFleur, M.W., D’Andrea J.M., D.G. Patterson, Streeter I.S.L., et al. In Vivo CRISPR Screening Reveals CHD7 as a Positive Regulator of Short-lived Effector Cells. J Immunol 15 November 2024; 213 (10): 1528–1541. https://doi.org/10.4049/jimmunol.2400213
- LaFleur, M.W., …, Streeter I.S.L, et al. A STUB1–CHIC2 complex inhibits CD8+ T cells to restrain tumor immunity. Nat. Immunol (2025). https://doi.org/10.1038/s41590-025-02231-6
- LaFleur M.W, …, Streeter I.S.L, et al. (Pending). Methods and compositions for treating cancers by modulating the expression and/or activity of STUB1. U.S. Patent No. PCT/US23/73178. U.S. Patent and Trademark Office.
Education
- MASTERS, Data Science | Harvard University, Extension School
- DATA SCIENCE CERTIFICATE | Harvard University, Extension School
- MASTERS, Cellular and Molecular Biology | University of New Haven
- BACHELORS, Biology | University of New Haven
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